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These
images were obtained via Kodak Photo CD, from the high resolution
Photo CD file (1536 x 1024 pixels, 4 Mb), and edited in Adobe
Photoshop. The original image was captured by photographing the
section on Kodachrome 35 mm film, using our specially designed
slide holder and illuminator, and a 42 mm Leitz Summar Macro lens
on a Pentax 35 mm SLR camera with a bellows extension for the
lens (see Methods).
This image was used to test possible image degradation through repetitive file compression and decompression.The upper image never underwent file compression; the lower image was processed through 20 cycles of compression and decompression, using the JPEG compression module in the PICT file Save menu of Photoshop, at the HIGH QUALITY level. For printing, the composite image was resized in Photoshop to 200 ppi and 8 Mb. Excellent prints were then produced on the Kodak XL 7700 dye sublimation printer, by DLM Imaging, Madison, WI. This is a horizontal section of the brain of a Tasmanian pademelon or Rufous wallaby (Thylogale billardieri) stained for myelin sheaths (Weil stain using hematoxylin).
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