Figure 1: Desktop Scanner
These images were obtained from directly scanning the tissue on the La Cie desktop Silverscanner II at 400 pixels per inch (ppi) (2400 x 3200 pixels, 3 Mb).
Both images were edited in Adobe Photoshop, and were assembled into the composite image presented here.
For printing, the composite image was then resized in Photoshop to 200 ppi and 8 Mb. Excellent prints were then produced on the Kodak XL 7700 dye sublimation printer, by DLM Imaging, Madison, WI. The top image is a sagittal section stained for cell bodies. The bottom image is an adjacent section stained for myelin sheaths.
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Figure 2: Desktop Scanner
These images were obtained from directly scanning the tissue on the La Cie desktop Silverscanner II at 400 pixels per inch (ppi) (2400 x 3200 pixels, 3 Mb).
The lower image was labeled in Adobe Photoshop, and assembled with the original unlabeled image into the composite image for printing.
For printing, the composite image was then resized in Photoshop to 200 ppi and 8 Mb. Excellent prints were then produced on the Kodak XL 7700 dye sublimation printer, by DLM Imaging, Madison, WI. The tissue is a fiber stained coronal section through the thalamus of a human brain.
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Figure 3: Compression Test
These images were obtained via Kodak Photo CD, from the high resolution Photo CD file (1536 x 1024 pixels, 4 Mb), and edited in Adobe Photoshop. The original image was captured by photographing the section on Kodachrome 35 mm film, using our specially designed slide holder and illuminator, and a 42 mm Leitz Summar Macro lens on a Pentax 35 mm SLR camera with a bellows extension for the lens (see Methods).
This image was used to test possible image degradation through repetitive file compression and decompression.The upper image never underwent file compression; the lower image was processed through 20 cycles of compression and decompression, using the JPEG compression module in the PICT file Save menu of Photoshop, at the HIGH QUALITY level.
For printing, the composite image was resized in Photoshop to 200 ppi and 8 Mb. Excellent prints were then produced on the Kodak XL 7700 dye sublimation printer, by DLM Imaging, Madison, WI. This is a horizontal section of the brain of a Tasmanian pademelon or Rufous wallaby (Thylogale billardieri) stained for myelin sheaths (Weil stain using hematoxylin).
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Figure 4: Photo CD
These images were obtained via Kodak Photo CD. The upper image (k hr 200) is from the high resolution Photo CD file (1536 x 1024 pixels, 4 Mb), and edited in Adobe Photoshop. The lower image used the same procedures but is from the base Photo CD file (768 x 512 pixels, 1 Mb).
The original image was captured by photographing a section of the brain of a Tasmanian pademelon or Rufous wallaby (Thylogale billardieri) on Kodachrome 35 mm film using our specially designed slide holder and illuminator, and a 42 mm Leitz Summar Macro lens on a Pentax 35 mm SLR camera with bellows extension for the lens (see Methods). Horizontal section stained with Thionine (Nissl Method).
For printing, the composite image was then resized in Photoshop to 200 ppi and 8 Mb. Excellent prints were then produced on the Kodak XL 7700 dye sublimation printer, by DLM Imaging, Madison, WI. Thylogale billardieri Tasmanian pademelon or Rufous wallaby Horizontal section Nissl stain (thionine).
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Figure 5: Digital Scanning Camera
(Leaf Lumina)
This image was prepared for us by Calumet Photographic, Inc., of Bensenville, IL, as an example of the quality of image that can be captured using the Leaf Lumina scanning digital camera. Calumet demonstrated the Lumina for us by scanning a coronal section stained for cell bodies (thionine stain, Nissl method) of a brain of a Rhesus monkey (Macaca mulatta).
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Figure 6: Photo CD vs Desktop Scanner
The images in this figure compare the results of photographing a brain section on 35 mm Kodachrome film and processing through Photo CD, with those from directly scanning the tissue on the La Cie desktop Silverscanner II.
The original Photo CD image was captured by photographing on Kodachrome 35 mm film using our specially designed slide holder and illuminator, and a 80 mm Leitz Summar Macro lens on a Pentax 35 mm SLR camera with bellows extension for the lens.
The Photo CD image is from the high resolution Photo CD file (1536 x 1024 pixels, 4 Mb); that from the scanner was scanned at 400 pixels per inch (ppi) (2400 x 3200 pixels, 3 Mb). Both images were edited in Adobe Photoshop, assembled into the composite image.
The tissue is a horizontal section stained for cell bodies (Thionine stain, Nissl method).
For printing, the composite image was then resized in Photoshop to 200 ppi and 8 Mb. Excellent prints were then produced on the Kodak XL 7700 dye sublimation printer, by DLM Imaging, Madison, WI.
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